Testate Amoebae –
Amoebozoa (Part), Stramenopiles (Part), Rhizaria (Part)

Observations on Difflugia brevicolla

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Taxonomic tree according to Lahr et al., 2019 (https://doi.org/10.1016/j.cub.2019.01.078)
and González-Miguéns et al., 2022 (https://doi.org/10.1016/j.ympev.2022.107557)

Amorphea Adl et al., 2012
Amoebozoa Lühe, 1913, renewed definition Cavalier-Smith, 1998
● Tubulinea Smirnov et al., 2005
●● Elardia, Kang et al., 2017
●●● Order Arcellinida Kent, 1880
●●●● Suborder Glutinoconcha Lahr et al., 2019
●●●●● Infraorder Longithecina Lahr et al., 2019
●●●●●● Family Difflugiidae Wallich, 1864
●●●●●●● Genus Difflugia Leclerc, 1815

Difflugia brevicolla Cash & Hopkinson, 1909

Most likely ID: n.a.

Basionym: n.a.

Add’l Synonyms: n.a.

Sampling date 06/2025. Scale bars indicate 50 µm (1), 25 µm (2).

Two images. Whole tests in side view and view of the aperture at an oblique angle.

Please click on < or > on the image edges or on the dots at the bottom edge of the images to browse through the slides!

Place name: Moor called Schwemm near Walchsee (Tyrol, Austria)
Latitude: 47.657707 Longitude: 12.299540 Elevation: 669 m

Microscope Zeiss Axioplan, camera Olympus OM-D M5 MKII. DOF images.

Sampling date 06/2025. Scale bars indicate 100 µm.

Two images. Whole tests in apertural view and view of the aperture at an oblique angle.

Please click on < or > on the image edges or on the dots at the bottom edge of the images to browse through the slides!

Place name: Moor called Schwemm near Walchsee (Tyrol, Austria)
Latitude: 47.657707 Longitude: 12.299540 Elevation: 669 m

Stereo microscope Olympus SZX16, camera Olympus OM-D M5 MKII. DOF images.

Six images.

First and second:	Complete tests. In addition to the many diatom frustules incorporated into the amoeba shells, uredospores (fruiting bodies of rust fungi, arrowhead) and cysts of chrysophytes (double arrowhead) are also found. Scale bars indicate 50 µm.
Third:	Detail view. Chrysophyte cyst and diatom frustules. Scale bar indicates 2 µm.
Fourth:	Detail view. Uredospores and diatom frustules. Scale bar indicates 5 µm.
Fifth:	Detail. During the preparation, scales of Centroplasthelida (genus Acanthocystis) were deposited on the shell. Scale bars indicate 2 µm.
Sixth:	Detail. View into the aperture of the shell. Scale bars indicate 2 µm.

Please click on < or > on the image edges or on the dots at the bottom edge of the images to browse through the slides!

Place name: Moor called Schwemm near Walchsee (Tyrol, Austria)
Latitude: 47.657707 Longitude: 12.299540 Elevation: 669 m

Tescan Clara SEM, © Wolfgang Bettighofer/Jürgen Stampfl

Uredospores from the water sample used above

Sampling date 06/2025. Scale bars indicate 25 µm.

Four images. Top view and optical cross-sections through uredospores (fruiting bodies of rust fungi).

Please click on < or > on the image edges or on the dots at the bottom edge of the images to browse through the slides!

Place name: Moor called Schwemm near Walchsee (Tyrol, Austria)
Latitude: 47.657707 Longitude: 12.299540 Elevation: 669 m

Microscope Zeiss Axioplan, camera Olympus OM-D M5 MKII. DOF images.

© Wolfgang Bettighofer,
images under Creative Commons License V 3.0 (CC BY-NC-SA).
For permission to use of (high resolution) images please contact postmaster@protisten.de.

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Observations on Difflugia brevicolla

Uredospores from the water sample used above

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